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. 2008 May;19(5):1883–1892. doi: 10.1091/mbc.E07-12-1230

Figure 3.

Figure 3.

DRhoGEF2 overexpression results in the formation of deep segmental grooves. All embryos express DRhoGEF2-RE driven by prd-Gal4 (A–E′), twi-Gal4 (F–H), or pnr-Gal4 (I), and they are stained for DRhoGEF2 (green), F-actin (red), and En (blue). (A and A′) stage 10: parasegmental grooves form prematurely in DRhoGEF2-overexpressing segments anterior to the En domain. (B and B′) Mid-stage 12: Cells contract and segmental grooves have formed prematurely in DRhoGEF2-overexpressing segments posterior to the posterior-most en-expressing cell. (C and C′) Late stage 12: groove founder cells overexpressing DRhoGEF2 do not elongate along their apical-basal axis. Segmental grooves are significantly deeper in DRhoGEF2-overexpressing segments. (D and D′) Stage 13: wild-type segments start to regress, whereas grooves persist in DRhoGEF2-overexpressing segments. (E and E′) Stage 14: wild-type grooves have regressed. DRhoGEF2-overexpressing grooves persist often showing reversed asymmetry. (F–H) DRhoGEF2-overexpression in the mesoderm does not influence segmental groove formation. Stages: 11 (F), 13 (G), and 15 (H). (I) Stage 15: DRhoGEF2 overexpression in the dorsal epidermis but not in underlying tissues results in deep segmental grooves. A–D and F–H show ventrolateral views; E and E′ show ventral views. I shows a dorsolateral view. Bar, 10 μm.