Figure 2.
Anterograde transport of Atg9 is impaired in the arp2-1 mutant. (A) The atg1Δ and arp2-1 atg1Δ strains chromosomally-tagged with Atg9-3xGFP and RFP-Ape1, and carrying a centromeric plasmid (pATG1ts(414)) encoding a temperature-sensitive allele of ATG1 were grown to mid-log phase at 24 and 37°C and visualized by fluorescence microscopy. Arrows mark the site of the PAS. DIC, differential interference contrast. Scale bar, 2 μm. (B) Quantification of the Atg9-3xGFP and RFP-Ape1 colocalization from (A); colocalization was defined as one Atg9-3xGFP dot overlapping with the RFP-Ape1 signal. Approximately 50 cells expressing RFP-Ape1 were examined. The number of cells (40) where Atg9-3xGFP and RFP-Ape1 colocalized in the atg1ts strain at 24°C was set to 100%. The values represent mean and standard deviation for 3 independent experiments.