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Localization of overexpressed tagged-FBXO25 in HEK293H cells. (A) HEK293H cells transfected with EGFP/HA-FBXO25-FLAG were fixed and immunostained with anti-NPM antibodies. (B) Western blotting analysis of transiently expressed EGFP-FBXO25 protein. Approximately 50 μg of protein from the nontransfected (NT) and transfected cells (T) lysates were subjected to SDS-PAGE, transferred onto nitrocellulose membranes and probed with monoclonal anti-GFP antibodies.
