Figure 7.

(A) Double labeling confocal experiments for the detection of FBXO25 and proteasomes in HeLa cells. (A) Proteasomes are accumulated in domains resembling clastosomes (Aiii, inset). (B) Proteasomes show a diffuse nucleoplasmic pattern. (C) Density and colocalization of FANDs and clastosomes in nuclei of HeLa cells as determined by counting 100 cells in each of four microscopic-slides. (D) Confocal analysis of cells double-labeled with antibodies against FBXO25 and Skp1 (Ci–Ciii). The labeled proteins in each panel are indicated in the top left and right of each panel in the respective color.