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. 2008 May;19(5):2179–2192. doi: 10.1091/mbc.E07-11-1155

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© 2008 by The American Society for Cell Biology

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Figure 5. — Changes in CMA lysosomal components in Atg5^−/− cells under basal conditions. (A) Homogenate (HOM) and lysosomes (LYS) isolated from wild-type and Atg5^−/− mouse embryonic fibroblasts maintained in the presence of serum were subjected to SDS-PAGE and immunoblotted for the indicated proteins. (B) Lysosomal membranes (MEMB) and matrices (MTX) isolated after hypotonic shock and centrifugation of intact lysosomes were processed as described in A. Bottom of A and B, densitometric quantification of two to three immunoblots. Values are expressed as -fold increase in lysosomes isolated from Atg5^−/− cells compared with wild type, which was assigned an arbitrary value of 1 (*p < 0.01, **p < 0.001 compared with WT cells).