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Deletion of ATG29 in atg11Δ cells affects PAS localization of Atg proteins. Localization of GFP-tagged Atg17, Atg29, and Atg31 was analyzed in the indicated atg mutant cells. Similarly, localization of Atg2 and Atg5 was observed. Rapamycin-treated (3 h) cells were observed by fluorescence microscopy. The percentages of cells with fluorescent dot signals at the PAS were determined. Bar, 5 μm.
