Table 1.
Yeast strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| SEY6210 | MATα ura3 leu2 his3 trp1 lys2 suc2 | Robinson et al. (1988) |
| TMK4 | SEY6210 atg29Δ::kanMX | Kawamata et al. (2005) |
| TMK174 | SEY6210 ATG29-myc-kanMX | This study |
| PJ69-4A | MATa his3-200 leu2-3,112 trp1-901 ura3-52 gal4Δ | |
| gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ | James et al. (1996) | |
| TMK327 | PJ69-4A atg29Δ::kanMX | This study |
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Giaever et al. (2002) |
| TMK181 | BY4741 atg29Δ::kanMX | Kawamata et al. (2005) |
| TMK190 | SEY6210 ATG29-GFP-kanMX | Kawamata et al. (2005) |
| TMK214 | TMK190 atg17Δ::his3MX | This study |
| TMK400 | TMK190 atg11Δ::LEU2 | This study |
| TMK200 | TMK190 atg1Δ::his3MX | This study |
| TMK464 | TMK400 atg17Δ::URA3 | This study |
| TMK553 | BY4741 ATG29-GFP-kanMX | This study |
| YKY163 | TMK553 atg11Δ::LEU2 atg31Δ::natMX | This study |
| TMK607 | TMK400 atg1Δ::his3MX | This study |
| TMK680 | TMK400 atg13Δ::URA3 | This study |
| TMK698 | TMK400 atg2Δ::HIS3 | This study |
| TMK728 | TMK400 atg9Δ:: his3MX | This study |
| TMK730 | TMK400 atg14Δ:: his3MX | This study |
| YKY161 | BY4741 ATG31-GFP-kanMX atg11Δ::LEU2 | This study |
| YKY157 | YKY161 atg17Δ::HIS3 | This study |
| YKY159 | YKY161 atg29Δ::natMX | This study |
| YKY155 | BY4741 ATG31-GFP-kanMX atg11Δ::URA3 atg1Δ::LEU2 | This study |
| YKY173 | YKY161 atg13Δ::URA3 | This study |
| YKY6 | BY4741 ATG17-GFP-his3MX | Kabeya et al. (2007) |
| TMK672 | YKY6 atg11Δ::kanMX atg1Δ::LEU2 | This study |
| TMK576 | BY4741 ATG17-GFP-kanMX | This study |
| TMK580 | TMK576 atg1Δ::LEU2 | This study |
| TMK600 | TMK576 atg29Δ::natMX | This study |
| TMK625 | TMK576 atg11Δ::LEU2 | This study |
| TMK623 | TMK625 atg29Δ::natMX | This study |
| YKY179 | TMK625 atg31Δ::natMX | This study |
| TMK686 | TMK625 atg13Δ::URA3 | This study |
| TMK696 | TMK625 atg2Δ::HIS3 | This study |
| TMK725 | TMK625 atg9Δ::his3MX | This study |
| TMK727 | TMK625 atg14Δ::his3MX | This study |
| TMK689 | BY4741 ATG2-GFP-kanMX atg11Δ::LEU2 | This study |
| TMK617 | TMK689 atg29Δ::natMX | This study |
| TMK584 | BY4741 ATG2-GFP-kanMX atg29Δ::natMX | This study |
| TMK690 | BY4741 ATG5-GFP-kanMX atg11Δ::LEU2 | This study |
| TMK618 | TMK690 atg29Δ::natMX | This study |
| TMK588 | BY4741 ATG5-GFP-kanMX atg29Δ::natMX | This study |
| TMK688 | BY4741 ATG1-GFP-kanMX atg11Δ::LEU2 | This study |
| TMK616 | TMK688 atg29Δ::natMX | This study |
| TMK731 | TMK688 atg13Δ::URA3 | This study |
| TMK732 | TMK688 atg17Δ::URA3 | This study |
| YKY175 | TMK688 atg31Δ::natMX | This study |
| TMK695 | BY4741 ATG13-GFP-kanMX atg11Δ::LEU2 | This study |
| TMK621 | TMK695 atg29Δ::natMX | This study |
| TMK765 | TMK695 atg1Δ::his3MX | This study |
| TMK766 | TMK695 atg17Δ::his3MX | This study |
| YKY177 | TMK695 atg31Δ::natMX | This study |
| OND88 | W303-1B pho8::pho8Δ60 | This study |
| YYK762 | OND88 atg11Δ::LEU2 | This study |
| YYK865 | SEY6210 ATG29-YFP-his3MX atg8Δ::TRP1 atg11Δ::LEU2 | This study |
| BJ2168 | MATa leu2 trp1 ura3-52 prb1-1122 prc1-407 pep4-3 | Yeast Stock Center |
| YYK806 | BJ6218 HA-ATG1 atg29Δ::kanMX | This study |
| YYK850 | BJ2168 HA-ATG1 ATG29-myc-natMX | This study |
| YYK868 | YYK850 atg11Δ::LEU2 | This study |
| YYK870 | YYK850 atg11Δ::LEU2 atg17Δ::kanMX | This study |
| YYK872 | YYK850 atg11Δ::LEU2 atg31Δ::kanMX | This study |
| YYK874 | BJ2168 HA-ATG1 ATG31-myc-hphMX | |
| atg11Δ::LEU2 atg29Δ::kanMX | This study | |
| YYK878 | BJ2168 HA-ATG1 ATG31-myc-hphMX | |
| atg11Δ::LEU2 atg17Δ::URA3 atg29Δ::kanMX | This study |