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Paraformaldehyde fixation does not remove HMGN from interphase chromatin. In fixed cells (A and B), HMGNs and DNA were detected by immunofluorescence as in Figure 1. In Living cells (C and D), HMGN-YFPs were directly visualized through YFP fluorescence, and DNA was stained with Hoechst 33342. The graphs show relative intensity of each signal along the white arrows and localization profiles of DNA and HMGNs in fixed and live cells. Note that in all cells the DNA and HMGNs colocalize.
