Skip to main content
. 2008 May;19(5):2083–2091. doi: 10.1091/mbc.E07-10-1088

Figure 5.

Figure 5.

Expression of HURP-C displaced the endogenous HURP from the mitotic spindle and phenocopied HURP depletion. (A) Maximum projections from deconvolved z-stacks of representative HeLa cells transfected with GFP or GFP-HURP-C and stained for the endogenous HURP using an antibody generated against HURP-N (red), GFP (green), and DNA (blue). Scale bar, 5 μm. (B and C) Maximum projection from deconvolved z-stacks of representative GFP-HURP-C–transfected HeLa cells stained for Hec1 (Alexa 680, shown as green), β-tubulin or CREST (Alexa 594, shown as red), and DNA (blue). Insets show single z-slices of the boxed regions. Scale bar, 5 μm. (D) Interkinetochore distance was quantified based on Hec1 and CREST staining for 40 kinetochore pairs in 8 HeLa cells. Prometa, prometaphase. *p < 8 × 10−8 (one-tailed t test). Error bars, SE. (E) Maximum projection from deconvolved z-stacks of GFP-HURP-C–transfected HeLa cells stained for Hec1 (green), Mad2 or BubR1 (red), and DNA (blue). Insets show single z-slices of the boxed regions. Scale bar, 5 μm. (F) Mad2 and BubR1 signals on 60 kinetochores from 12 prometaphase (prometa) or metaphase cells were quantified in control and GFP-HURP-C–transfected HeLa cells. For the unaligned chromosomes in GFP-HURP-C–expressing cells, one pair of kinetochores in each of eight cells with an unaligned chromosome was quantified and then a mean and SE were calculated from the 16 kinetochores. *p < 2 × 10−4; **p < 2 × 10−6; +p < 3 × 10−8 (one-tailed t test). Error bars, SE.