Figure 1.

Structural features, phylogenetic relationship, and tissue distribution of USP30. (A) A Kyte–Doolittle hydropathy plot of the amino acid sequence of human USP30. The plot was constructed by using the GENETYX program (Software Development, Tokyo, Japan). The putative TM region is shaded. (B) Schematic structure of human USP30. The putative TM region and DUB catalytic domain are indicated in black and gray, respectively. The conserved Cys (Cys 77) and His (His 452) residues are indicated by C and H, respectively. (C) A phylogenetic analysis of human USP30 with its homologues and the yeast (S. cerevisiae) UBP members. The tree was constructed by the neighbor-joining method by using the ClustalW computer program. The scale bar represents a genetic distance of 0.5-amino acid substitution per site. The GenBank accession numbers are as follow: human USP30, CAE51936; rat (Rattus norvegicus) USP30, NP_001100623; chicken (Gallus gallus) USP30, XP_415187; western clawed frog (Xenopus tropicalis) USP30, AAI35926; zebrafish (Danio rerio) USP30, CAM16313; fruit fly (Drosophila melanogaster) USP30 #1 (CG3016-PA), NP_572274; fruit fly USP30 #2 (GA15687-PA), XP_001355317; nematode (Caenorhabditis elegans) USP30 (Y67D2.2), NP_497422; UBP1p, NP_010161; UBP2p, NP_014767; UBP3p, NP_011078; UBP4p, NP_010354; UBP5p, NP_011071; UBP6p, NP_116665; UBP7p, NP_012110; UBP8p, NP_013950; UBP9p, NP_011024; UBP10p, NP_014213; UBP11p, NP_013024; UBP12p, NP_012338; UBP13p, NP_009486; UBP14p, NP_009614; UBP15p, NP_014033; and UBP16p, NP_015253. (D) A Northern blot of 20 μg of total RNA prepared from the indicated rat tissues was hybridized with specific probes for USP30 (top) and β-actin (bottom).