Figure 3.

PI-3P increases AP-3 recruitment. (A) Liposomes with LIMP-II tails containing different or no PIPs were incubated with cytosol (3 mg/ml), GTPγS, and phosphatase inhibitors. AP-3σA/B bound to liposomes was detected after SDS-PAGE and Western blotting. (B) Liposomes with or without LIMP-II tails and either PI-3P or PI-4P or no PIP were incubated with cytosol (3 mg/ml), GTPγS, and phosphatase inhibitors. AP-1γ, AP-2α, AP-3σA/B, COP-Iβ, and ARF-1 bound to liposomes were detected after SDS-PAGE and Western blotting. The AP-3σA/B signal was quantified.