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Requirements for stabilization of AP-3, septins, and Rab7 on liposomes. Liposomes with or without LIMP-II and with or without PI-3P were incubated with cytosol and GTPγS. After incubation, AP-3σA/B, AP-1γ, ARF-1, Rab7, and Septin 7 were analyzed by Western blotting by using specific antibodies. The recruitment of these markers was quantified (black bar, LIMP-II and PI-3P; dark gray bar, LIMP-II no PI-3P; light gray bar, PI-3P and no LIMP-II; and white bar, no LIMP-II and no PI-3P).
