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. 2008 May;19(5):2003–2013. doi: 10.1091/mbc.E07-09-0894

Figure 2.

Figure 2.

Stathmin tubulin-sequestering activity is necessary to stimulate cell motility. (A) Migration through FN-coated FluoroBloks of HT-1080 cells transiently transfected with the indicated EGFP vectors. Data are expressed as the percentage of stimulation with respect to the surrounding untransfected cells and represent the mean (±SD) of three independent experiments, in which at least 100 transfected cells were counted. (B) Western blot analysis of soluble (S) and polymerized (P) fractions of tubulin in HT-1080 cells transiently transfected with the indicated pFLAG-vectors and adhered to FN for 60 min. The expression of vinculin (loading control), β-tubulin, polyglutamylated-tubulin, and stathmin is shown. (C) Confocal images of HT-1080 cells transiently transfected with the indicated EGFP constructs (green) and stained with phalloidin or acetylated tubulin (red). In the bottom panels, only the acetylated tubulin staining is shown. Yellow arrows indicate the transfected cells.