Figure 3.

GEF-H1 depletion abolishes nocodazole-induced contraction in PMA-induced differentiated D2 cells. Two stable shRNA-expressing erythroblastoma D2 cell lines, control LacZ^shRNA, and GEF-H1–depleted GEF-H1₂₁₇^shRNA were treated with 32 nM PMA in serum-free RPMI medium for 2 h to induce adhesion and differentiation. Cells were then treated with or without 3.3 μM nocodazole for 1.5 h. Representative PMA-induced attached (PMA-att) and nocodazole-treated adherent (PMA-att+Noc) D2 cells were observed by phase-contrast microscopy and are shown as indicated.