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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: J Invest Dermatol. 2007 May 31;127(11):2645–2655. doi: 10.1038/sj.jid.5700890

Figure 3. TNF-α affects the mechanical response of the fibroblast collagen constructs.

Figure 3

The mechanical response of FPCL treated with TGF-β1 (1 ng/ml), TNF-α (1 ng/ml), and a combination of TGF-β1 and TNF-α were measured using compressive indentation assay. In the indentation assay, normal fibroblasts (3 × 105 cells/cm3) were seeded in collagen I matrix (1.5 mg/ml) and induced with cytokines for 72 hours. The constructs underwent indentation measurements. (a) Schematic of the mechanical test and equation used for calculating Young’s modulus. (b) The data are expressed as Young’s modulus for each experimental group.