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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: J Invest Dermatol. 2007 May 31;127(11):2645–2655. doi: 10.1038/sj.jid.5700890

Figure 5. TNF-α affects α-SMA mRNA stability.

Figure 5

Adult human dermal fibroblasts were grown in monolayer to confluence and serum-starved for 16 hours before induction with TGF-β1 (1 ng/ml) or TGF-β1 (1 ng/ml) plus TNF-α (1 ng/ml) for 24 hours. At the end of 24 hours, actinomycin D (2.5 μg/ ml) was added and total RNA was harvested at different time points for real-time PCR analysis. The graph represents a ratio of α-SMA and GAPDH mRNA versus time from three independent experiments with SD.