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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: J Invest Dermatol. 2007 May 31;127(11):2645–2655. doi: 10.1038/sj.jid.5700890

Figure 7. TNF-α decreases TGF-β1 induction of α-SMA expression in a JNK-dependent pathway.

Figure 7

(a) Human dermal fibroblasts were grown in monolayer to confluence and serum starved overnight followed by induction with TNF-α. Total protein was harvested at indicated time and Western blot analysis was performed for phosphorylated JNK and JNK proteins. (b) FPCL was prepared in a final cell density of 300,000 cells/ml plus collagen concentration of 1 mg/ml and induced with different cytokine combinations with or without SB202190 and SP600125 inhibitors for 96 hours. Cell lysates were analyzed for α-SMA and GAPDH expression using Western blot. (c) The graph represents mean α-SMA expression as a ratio of GAPDH obtained by densitometric analysis from three independent experiments.