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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: J Invest Dermatol. 2007 May 31;127(11):2645–2655. doi: 10.1038/sj.jid.5700890

Figure 8. TNF-α inhibits Smad3 phosphorylation in normal human dermal fibroblasts.

Figure 8

To test whether TNF-α affects the TGF-β1 Smad signaling pathway, human dermal fibroblasts were grown in monolayer and induced with TGF-β1 (1 ng/ml) or TGF-β1(1 ng/ml) combined with TNF-α (1 ng/ml). Cell lysates were analyzed for phosphorylated Smad3 and GAPDH using Western blot analysis.