Skip to main content
. Author manuscript; available in PMC: 2008 Jun 1.
Published in final edited form as: Gastroenterology. 2007 Apr 13;132(7):2422–2437. doi: 10.1053/j.gastro.2007.03.101

Figure 8.

Figure 8

Effects of TxA on NCM460 cells. (A) Real-time quantitative PCR of PAR2 levels. Results of 3 experiments are shown. After 120 minutes of exposure to TxA, PAR2 levels were increased and remained increased even after 360 minutes. (B) Real-time quantitative PCR of trypsin IV levels. Results of 3 experiments are shown. After 120 minutes of exposure to TxA, trypsin IV levels were increased and remained increased even after 360 minutes. (C) Ca2+ mobilization in response to PAR2 AP or vehicle (1/1300 dilution of 0.05 mol/L Tris in NCM culture medium) in cells pretreated with 5 nmol/L or 5 μmol/L TxA. Ca2+ responses to PAR2-AP (10−5 mol/L) are expressed as a 340/380 ratio (peak baseline; n = 38−45 cells). TxA significantly enhanced responses to PAR2-AP. *P < .05 between control or 0 minutes and TxA-exposed cells. †P = .055 between control or 0 minutes and TxA-exposed cells.