Figure 1.

Inhibiting amyloid formation. The extent of TTR amyloid fibril formation (37°C) in the absence and presence of various concentrations of Flu probed by OD measurements at 330 nm (shown) and by quantitative Congo red binding (not shown, data virtually identical) (24). The inhibition of wild-type (pH 4.4), V30M (pH 5.0), and L55P (pH 5.0) TTR by variable concentrations of Flu are shown with the errors associated with these measurements. The pHs chosen are those associated with maximal amyloid fibril formation. The table (Inset) provides additional information including pH_m, the pH midpoint of the tetramer monomer equilibrium, and the IC₅₀, the concentration of Flu required for reduction of amyloid fibril formation to 50% of its maximum value.