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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: J Med Microbiol. 2007 Apr;56(Pt 4):557–562. doi: 10.1099/jmm.0.46891-0

Fig. 1.

Fig. 1

(a) Organization of the katA gene in the H. hepaticus genome and DNA constructs for generating the katA mutant. A 1200 bp fragment of the katA gene was amplified using the katAF/katAR primer pair and cloned into pGEM-T. A CmR cassette was then inserted within the katA fragment at the HindIII restriction site and the plasmid pGEM-katA ∷ Cm was used for transformation of wild-type H. hepaticus to create the katA mutant. (b) Agarose gel analysis of PCR products using primers katAF/katAR. Lanes: 1, wild-type H. hepaticus; 2, katA mutant; M, DNA size standard.