Fig. 3.

a Disulfide reduction of rBmTCTP by Trx system. rBmTCTP was added to a reaction mixture containing 3 μM of TR and 3 μM of Trx in TE buffer containing 400 μM NADPH. Reference controls included reaction mixtures without the addition of Trx or without the addition of TR. Samples were incubated at room temperature, and oxidation of NADPH was monitored spectrophotometrically at 340 nm. Readings were taken every 30 s from 0.5 to 10 min, and the reference readings were subtracted from the test reaction to determine NADPH consumption. Results are representative of three similar experiments. b Overexpression of rBmTCTP conferred resistance to H₂O₂ damage in E. coli cells. Cells transfected with rBmTCTP or blank vectors were serially diluted from 1:10 to 1:100 and plated on to LB agar plates containing 1.2 mM H₂O₂. After incubation for 12 h at 37°C, growth of bacterial cells was evaluated. Results show that cell transfected with the blank vector failed to grow under these conditions, whereas cells transfected with rBmTCTP showed growth even up to a dilution of 1:50 in the presence of H₂O₂. Data represent results from one of two similar experiments