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. Author manuscript; available in PMC: 2008 May 5.
Published in final edited form as: Cancer Lett. 2006 Oct 9;249(2):148–156. doi: 10.1016/j.canlet.2006.08.008

Fig. 2.

Fig. 2

Expression of hPMS2-E705K in transiently transfected mouse cells. (A) Representative immunoblot of whole cell lysates (WCLs) of MP1 cells transfected with hMLH1 (1 μg). hPMS2 (0.25 μg,), and/or hPMS2-E705K (0.25 μg) cDNAs, together with pCPB (1.25–1.5 μg) and pCNB (0.5–1.5 μg) empty vector DNAs to bring the total amount of DNA transfected to 3 μg. (B) Representative immunoblot of WCLs of MP1 cells transfected with 0.25–2 μg of hPMS2 or hPMS2-E705K cDNAs. (C) Quantitative analysis of PMS2 expression in MP1 cells transfected with increasing amounts of hPMS2 versus hPMS2-E705K cDNAs. Each point on the graph represents the average of 3–4 experiments consisting of duplicate transfections. Linear regression was performed on each data series.