Figure 3.

Inhibition of SPE7 Fv binding to immobilized DNP-Asn by various cross-reactants as measured by competitive ELISA. Noted are the abbreviated names of the various cross-reactants, the full names and structures of which are given in Table 2. Residual SPE7 binding to immobilized DNP-Asn was determined by measuring capture of SPE7 using anti-myc-HRP secondary antibody (see Materials and Methods). Binding, as a percentage of the binding observed in the absence of any inhibitor, was plotted against inhibitor concentration. IC₅₀s were determined as the concentration of ligand needed for 50% inhibition and are given in Table 2.