Table 2.

Structures and affinities of various SPE7 ligands

The immunizing hapten (indicated in bold), DNP derivatives, and several unrelated haptens are shown in the left column. In the right column, is shown alizarin and a series of related derivatives. Binding affinities were determined by fluorescence quenching,^a or by competitive ELISA with either DNP-Biotin^b or DNP-Asn-BSA^c (for details, see Materials and Methods). Those compounds which gave less than 50% inhibition in competition ELISAs at a concentration of 200 μM are indicated as having an affinity >200 μM, and are effectively considered to be nonbinding..