Table 3.
Values of equilibrium constants of dimerization (Kd) and kinetic parameters (k2) of dimer dissociation and association
| Enzyme | Number of experiments | Stock enzyme concentration (μM) | Assay enzyme concentration (nM) | Kd (nM) | K2 (10−4 sec−1) | τ1/2 (min) | k1 (103 M−1 sec−1) |
| MAV-PR(S) | 6 | 18.4 | 12–62 | 412 ± 122 | 7.47 ± 1.07 | 15.5 ± 2.2 | 1.81 ± 0.79 |
| MAV-PR(T) | 5 | 13–27 | 21–51 | 31.5 ± 3.3 | 7.81 ± 1.49 | 14.8 ± 2.8 | 24.8 ± 18.3 |
| HIV-1-PR(T) | 7 | 3–21 | 1–7 | 15.1 ± 4.3 | 6.78 ± 1.97 | 17.0 ± 5.0 | 44.9 ± 25.8 |
| HIV-1-PR(S) | 4 | 0.75–10 | 2–10 | 122 ± 28 | 12.1 ± 1.09 | 9.5 ± 0.8 | 10.0 ± 3.19 |
| MPMV-PR(T) | 4 | 2.78 | 4–19 | 13.5 ± 8.3 | 28.1 ± 10.8 | 4.1 ± 1.6 | 208.1 ± 207.9 |
Specific experimental conditions for each enzyme are indicated.