Figure 2.

Fibril formation (monitored by ThT fluorescence) as a function of TFE concentration. The curves represent the changes of fluorescence with TFE concentrations at pH 3.5 (filled circles) and pH 5.5 (open circles) after 18 h of incubation. ThT binding was monitored after removing an aliquot of the aggregation mixture (10 μL) and diluting it with 990 μL of 50 mM sodium phosphate, pH 6.0, containing 3 μM ThT. Fluorescence intensity was determined at 485 nm after excitation at 440 nm using a 96-well plate reader equipped with cutoff filters as previously described (Wood et al. 1996).