Figure 14.

SDS-PAGE analysis of susceptibility to tryptic digestion of native and allicin-modified TcAChE. SDS-PAGE was carried out under nonreducing conditions on 5%–15% acrylamide gradient gels. Enzyme samples (1 mg/mL) were digested with trypsin (1% [w/w]) at 23°C for 1 h. (Lane 1) Control, native TcAChE; (lane 2) trypsin-digested native TcAChE; (lane 3) TcAChE modified by treatment with 1 mM allicin at 22°C for 30 min; (lane 4) allicin-modified enzyme digested with trypsin; (lane 5) allicin-modified enzyme digested with trypsin in the presence of 1 mM MgCl₂; (lane 6) molecular weight markers.