Figure 5.

Molecular weight of the Notch ankyrin domain polypeptide at pH 6.5 determined by multiangle static light scattering. Static light scattering measurements were made in the presence (solid line, plus symbols) and absence (dashed line, open circles) of 10 mM MgCl₂. The chromatographic peaks (lines) were monitored by refractometry (right axis) and the weight-averaged molecular weights (left axis) are represented as points within the chromatogram. The downward curvature of the molecular weight distribution is likely to result from peak broadening between the scattering and refractive index detectors.