Table 2.

Effects of pH and magnesium ion concentration on the apparent molecular weight of the Notch ankyrin domain

pH	Magnesium concentration (mM)	Mw,obsa (daltons)
6.5	0	28,100 ± 140
	10	26,900 ± 720
	200	25,700 ± 180
7.0	0	30,400 ± 340
	10	28,500 ± 610
7.4	0	26,800 ± 820
	10	25,100 ± 450
8.0	0	25,700 ± 240
	10	26,700 ± 160
Mw,calcb		25,935

All samples contained 150 mM NaCl and 25 mM buffer as described in Materials and Methods.

^aMolecular weight determined by multi-angle static light scattering, as described in Materials and Methods. Reported errors are the root mean square deviation from at least three separate measurements.

^bMolecular weight calculated from primary sequence.