. 2003 Nov;12(11):2389–2403. doi: 10.1110/ps.03302003

Table 1.

Acrylamide quenching of tryptophan fluorescence

System	K_SV^a (M⁻¹)	k_q (×10⁻⁹)^b (M⁻¹s⁻¹)	f_a^c
Spectrin (native)	4.40 ± 0.04	1.29	0.40 ± 0.01
Spectrin (denatured)	8.60 ± 0.30	2.02	0.65 ± 0.04
NATA (water)	17.52 ± 0.30	5.84
NATA (8 M Urea)	13.42 ± 0.30	3.33

The concentration of spectrin and NATA used was 0.1 and 33 μM, respectively.

^a Calculated using equation 3. The quenching parameter shown represents mean ± standard error of three independent measurements while quenching data shown in Figure 6A ▶ are from representative experiments.

^b Calculated using mean fluorescence lifetimes from Table 3 and using equation 3.

^c Calculated using equation 4. The quenching parameter shown represents mean ± standard error of three independent measurements while quenching data shown in Figure 6B ▶ are from representative experiments.