Figure 4.

Selected antirhodopsin antibody consensus epitopes for mAbs with no rhodopsin photo-state preference, mapped to the surface of the dark rhodopsin x-ray structure. The top line of text in each panel is the antibody name followed by the mapping ID in parentheses that corresponds to the mapping numbers in Table 4. The second line of text is the (C)onsensus epitope "probe" sequence, and the third line contains the residues used in the (M)apping. Mapped epitope residues are ordered such that they follow a rainbow color scheme from red (residue one of the consensus epitope) to purple (and pink if there are 10 residues in the epitope, as also shown in Table 4). A two-dimensional connectivity map is shown below each image, with corresponding residues having the same colors as in the images above. Distance proximity constraints on rhodopsin for each mapping are shown in the two-dimensional connectivity map as curved black lines connecting non-contiguous rhodopsin residues. (A, B) Two views of the epitope of the B1gN mAb on the intradiskal surface from different angles, showing consensus epitope peptide SFVDFSNK that was mapped on rhodopsin as SFGDFSNKG (mapping 1 for this mAb from Table 4). (C) View of cytoplasmic mAb 4B4 consensus peptide EQQVSATAQ mapped on rhodopsin residues as EQQASATTQ (mapping 1 for this mAb from Table 4). (D) Proposed orientation of residues 235–244 of the C-3 loop of rhodopsin, based on the mapping scheme in C.