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Average distances and angles of docked ACh ligand in the anionic (tail) subsite
| No. of structures | dring86 (Å) | dring337 (Å) | dO202.1 (Å) | |
| Native | 958 | 4.50 | 5.13 | 3.82 |
| Zeroed-W86 | 771 | 4.69 | 4.75 | 4.14 |
| Zeroed-Y337 | 774 | 4.67 | 4.75 | 4.13 |
| Zeroed-E202 | 755 | 4.69 | 4.57 | 4.31 |
| Y337F | 778 | 4.86 | 4.73 | 4.11 |
| E202A | 614 | 5.02 | 4.57 | 5.03 |
