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Average distances and angles of docked ACh ligand in the anionic subsite
| No. of structures | dring86 (Å) | dring337 (Å) | dO202.1 (Å) | |
| Native | 958 | 4.50 | 5.13 | 3.82 |
| W86F | 659 | 5.58 | 4.70 | 3.93 |
| W86A | 655 | NA | 4.59 | 4.06 |
| Y337A | 148 | 5.00 | NA | 3.61 |
| E202A | 330 | 5.09 | 4.68 | NA |
| E202D | 554 | 5.19 | 5.41 | 3.96 |
