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The temperature dependence of enzyme activity was assayed by hydrolysis of the chromogenic substrate oNPC (ortho-nitrophenyl cellobioside) in 0.05 M Bis-Tris propane, 0.05 M ammonium acetate (pH 5.5) over 10 min at 25°C, 40°C, 50°C, and 60 °C. Data are shown for H. grisea Cel12A (filled squares) and T. reesei Cel12A (filled diamonds; Sandgren et al. 2003). It is expressed as a molar specific activity relative to T. reesei Cel12A at 40°C.
