Figure 2.

T3A induces STAT1 phosphorylation in the brain of neonatal mice following intracerebral inoculation. Two-day-old Swiss Webster mice were mock or T3A infected (1 × 10³ PFU, i.c.) and sacrificed at 4 or 8 days post infection. Whole-brain lysates were prepared from each animal and samples were then probed by Western blot for STAT1 phosphorylated at either Y701 or S727 (A). Nuclear and cytoplasmic brain fractions were prepared from identically treated neonates after sacrifice at 8 days post infection. Subcellular brain fractions from mockand T3A-infected animals were probed for pY701 and pS727 STAT1 (B). Images provide representative observations from a minimum of six animals sampled per treatment.