Table 1.
Transduction and toxicity of AAV serotypes on cultured neurons at 2 weeks post-transduction
| Serotype | Dose (GC) | Percent Transduction (GFP+/DAPI+) | Total Cells Counted (n) | Cultures (n) | Percent of transduced cells that are neurons (MAP2+GFP+/GFP+) | Total Cells Counted (n) | Cultures (n) |
|---|---|---|---|---|---|---|---|
| Naturally occuring | |||||||
| 1 | 2.5 × 1011 | 94.1 ± 1.9% | 739 | 4 | 79.6 ± 4.1% | 295 | 3 |
| 2 | 2.0 – 2.5 × 1011 | 83.8 ± 4.0% | 736 | 5 | 53.6 ± 9.0% | 533 | 4 |
| 5 | 2.5 × 1011 | toxic | 2 | - | |||
| 2.5 × 109 | 48.4 ± 9.4% | 1074 | 3 | 61.9 ± 17.7% | 227 | 3 | |
| 6 | 2.5 × 1011 | toxic | 3 | - | |||
| 6.7 × 1010 | toxic | 6 | - | ||||
| 2.5 – 5.0 × 109 | 72.1 ± 19.1% | 822 | 3 | 84.7 ± 10.4% | 264 | 3 | |
| 7 | 2.5 × 1011 | 88.0 ± 4.3% | 785 | 5 | 74.4 ± 12.8% | 392 | 4 |
| 8 | 2.5 × 1011 | 94.2 ± 1.9% | 599 | 5 | 63.2 ± 13.9% | 412 | 4 |
| 9 | 2.5 × 1011 | 79.5 ± 5.8% | 985 | 4 | 43.2 ± 2.2% | 320 | 3 |
| Engineered variants | |||||||
| 2.hu29R | 3.1 × 1011 | 0% | 3 | - | |||
| 6.1 | 5.0 × 1011 | 91.3 ± 1.7% | 491 | 3 | 64.1 ± 4.1% | 399 | 3 |
| 6.2 | 5.0 × 1010 | toxic | 3 | - | |||
| 6.1.2 | 5.0 × 1011 | 94.1 ± 2.8% | 443 | 3 | 85.1 ± 2.5% | 282 | 3 |
All vectors listed in this table encoded CMV-GFP. Vectors were added to cells at 7DIV; counts were performed at 21DIV.