Altered specificity of the triple-mutant H5 compared with wild-type KAN-1 H5 coexpressed with NA. Glycan microarray analysis of (A) wild-type or (B) triple-mutant HA purified after coexpression with NA was performed by a modification (18) of a previous technique (12) performed by Core H, Consortium for Functional Genomics, Emory University. Glycans with related linkages are grouped by color: selected glycoproteins (orange), predominantly α2,3-sialosides (yellow), α2,6-sialosides (green), α2,8 ligands (blue), or others (purple), as previously shown (table S3).