FIGURE 1.
Configuration of the double-supported membrane model system used in this work (A). Phase diagram (B) at 20°C for a ternary membrane (DOPC/DPPC/cholesterol) where the liquid-ordered/liquid-disordered (Lo + Ld, red line) coexistence region was adapted from Veatch and Keller (37). The yellow markers (I–IV) denote the compositions used in Figs. 2–6. The dotted lines indicate possible compositional alterations to (I ) and (II) assuming removal of DOPC only (purple), assuming approach toward a 1:1 ratio of DPPC to cholesterol (blue) or assuming removal of DPPC and DOPC with equal probability (orange).