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. 2008 Jan 30;94(10):3912–3923. doi: 10.1529/biophysj.107.122531

FIGURE 3.

FIGURE 3

Simulation of effects of 1 mM 3-O methyl D-glucose (3-OMG) and 20 mM urea and phloridzin on water flows into oocytes expressing SGLT1. (A) The simulated oocyte percentage volume change (solid line) superimposed on the observed experimental data obtained from Zeuthen et al. (23) (open circles). The first period starts upon exposure to isotonic 1 mM 3-OMG, the second upon addition of 20 mM hypertonic urea + 1 mM 3-OMG, the third upon removal of urea, the fourth upon isotonic removal of 3-OMG and addition of phloridzin (negligible tonicity), and the fifth period upon addition of 20 mM hypertonic urea + phloridzin. (B) The changes in compartmental 3-OMG during the five experimental periods shown in A. (C) The compartmental and extracellular changes in total osmolarity. (D) The separate water flow rates via the transporter, the membrane, and the total transmembrane flows. (E) The changes in vestibular and unstirred layer total osmolarity mOsm, superimposed on the water flow rates via the transporter and membrane using a 100× faster time base than in A and D.