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. Author manuscript; available in PMC: 2009 Mar 15.
Published in final edited form as: Biochem Pharmacol. 2007 Dec 4;75(6):1341–1347. doi: 10.1016/j.bcp.2007.10.037

Figure 2.

Figure 2

The ability of 2-MeSADP, and the caged 2-MeSADP (MRS2703) to activate the human P2Y1 receptor stably expressed in 1321N1 astrocytoma cells. Cells were loaded with 50 μl dye with 2.5 mM probenecid in each well and incubated for 45 min at room temperature. The agonists were tested without exposure to UV light. 2-MeSADP induced a concentration-dependent rise in [Ca2+]i levels, with an EC50 value of 2.03 ± 0.35 nM (n=3), similar to previously reported values [18].