Skip to main content
. Author manuscript; available in PMC: 2009 Mar 21.
Published in final edited form as: Cell. 2008 Mar 21;132(6):1025–1038. doi: 10.1016/j.cell.2008.01.030

Table 1.

skn-1 contributes to the lifespan extension resulting from reduced IIS.

Strain mean life span ± SEM 20°C (days) Median lifespan 75th percentile 20°C (days) 25th percentile 20°C (days) p value against control n Trials
N2 20.44 ± 0.3 21 23 19 - 90/90 1
daf-2 (e1368) 29.42 ± 0.3 31 31 29 <.0001a 91/91 1
daf-2 (e1370) 39.39 ± 0.4 39 43 37 <.0001a 88/88 1
daf-16 (mgDf47) 16.80 ± 0.3 17 19 15 <.0001a 50/50 1
daf-16 (mgDf47) ; daf-2 (e1370) 20.01 ± 0.1 21 21 19 <.0001b 77/80 1
skn-1(zu67) 15.60 ± 0.3 17 17 15 <.0001a 73/73 1
skn-1(zu129) 17.67 ± 0.2 17 19 17 <.0001a 90/90 1
skn-1(zu135) 17.70 ± 0.3 17 19 17 <.0001a 57/57 1
daf-2 (e1368); skn-1(zu67) 22.63 ± 0.5 23 25 21 <.0001c 60/60 1
daf-2 (e1368); skn-1(zu129) 22.25 ± 1.1 19 29 15 <.0001c 58/60 1
daf-2 (e1368); skn-1(zu135) 19.83 ± 0.6 19 23 17 <.0001c 58/59 1
daf-2 (e1370); skn-1(zu67) 38.60 ± 1.0 41 43 35 .0740b 60/60 1
daf-2 (e1370); skn-1(zu129) 27.64 ± 0.7 27 31 23 <.0001b 62/62 1
daf-2 (e1370); skn-1(zu135) 33.16 ± 0.6 39 43 33 .6016b 25/25 1
rrf-3(pk1426);control(RNAi) 19.53 ± 0.5 19 24 17 - 57/60 1
rrf-3(pk1426);skn-1(RNAi) 20.43 ± 0.6 21 24 15 .1531g 60/60 1
rrf-3(pk1426);skn-1a/c(RNAi) 20.27 ± 0.6 21 24 15 .2313g 59/60 1
rrf-3(pk1426);daf-2(e1368) control(RNAi) 38.62 ± 1.3 40 44 34 <.0001g 52/55 1
rrf-3(pk1426); daf-2(e1368) skn-1(RNAi) 25.83 ± 1.3 21 36 17 <.0001d 56/56 1
rrf-3(pk1426); daf-2(e1368) skn-1a/c (RNAi) 26.32 ± 1.3 24 36 19 <.0001d 57/58 1
N2 [rol-6]; control(RNAi) 20.1 ± 0.3 20 22 18 84/84 1
skn-1(zu67); control(RNAi) 14.6 ± 0.4 14 16 12 <.0001e 87/90 1
N2 [rol-6]; cyc-1(RNAi) 42.1 ± 0.9 42 45 40 <.0001e 32/34 1
skn-1(zu67); cyc-1(RNAi) 41.9 ± 1.2 45 47 38 .5594f 38/38 1
N2 [rol-6]; cco-1(RNAi) 31.52 ± 0.5 32 34 30 <.0001e 67/69 1
skn-1(zu67); cco-1(RNAi) 35.13 ± 0.5 36 38 34 <.0001f 46/46 1
Strain mean adult life span ± SEM 25°C (days) Median lifespan 75th Percentile 25°C (days) 25th percentile 25°C (days) p value against Control n No. Exp
rrf-3(pk1426); control(RNAi) 12.90 ± 0.4 13 15 11 - 99/100 2
daf-16(mgDf47) 11.04 ± 0.2 11 13 9 <.0001g 138/138 3
rrf-3(pk1426); akt1; akt-2(RNAi) 22.50 ± 0.7 22 28 17 <.0001g 120/138 2
daf-16(mgdf47); rrf-3(pk1426); akt1; akt-2(RNAi) 11.03 ± 0.3 11 13 10 <.0001h 123/123 3
rrf-3(pk1426);skn-1(zu67); control(RNAi) 7.20 ± 0.2 7 8 6 <.0001g 106/106 2
rrf-3(pk1426);skn-1(zu129);control(RNAi) 9.30 ± 0.4 8 12 7 <.0001g 100/100 2
rrf-3(pk1426);skn-1(zu135);control(RNAi) 12.00 ± 0.3 12 15 9 0.1012g 150/150 3
rrf-3(pk1426);skn-1(zu67); akt-1; akt-2(RNAi) 11.60 ± 0.6 11 17 6 <.0001h 104/104 2
rrf-3(pk1426);skn-1(zu129); akt-1; akt-2(RNAi) 20.40 ± 1.0 21 28 12 0.9400h 101/104 2
rrf-3(pk1426);skn-1(zu135); akt-1; akt-2(RNAi) 17.69 ± 0.6 17 22 13 <.0001h 110/110 3
N2; control(RNAi) 13.8 ± 0.3 14 16 12 - 110/114 2
N2; sgk-1(RNAi) 22.6 ± 0.4 23 25 21 <.0001e 110/395 2
skn-1(zu67); sgk-1(RNAi) 8.2 ± 0.2 8 10 7 <.0001i 128/128 2
skn-1(zu129); sgk-1(RNAi) 9.1 ± 0.2 8 11 7 <.0001i 185/186 2

Corresponds to the data in Figure 4C–H. SEM: standard error of the mean. 75th and 25th percentiles refer to the day at which 75% or 25% of the population is dead. n represents total number of animals dying of old age vs those in total experiment (combined between trials where appropriate). In the case of sgk-1(RNAi) the majority of exclusions are due to Egl/bag of worms phenotypes. p values were calculated as follows:

a

N2,

b

daf-2(e1370),

c

daf-2(e1368),

d

rrf-3(pk1426);daf-2(e1368) control(RNAi),

e

N2 control(RNAi),

f

N2 with corresponding RNAi,

g

rrf-3(pk1426) control(RNAi),

h

rrf-3(pk1426) akt-1;akt-2(RNAi),

i

sgk-1(RNAi). Individual experiments, an additional analysis of daf-2(e1368) and daf-2(e1370), and other supporting data are in Table S5.