Skip to main content
. Author manuscript; available in PMC: 2009 Mar 21.
Published in final edited form as: Cell. 2008 Mar 21;132(6):1025–1038. doi: 10.1016/j.cell.2008.01.030

Table 2.

Transgenic expression of SKN-1 extends lifespan.

Strain mean life span ± SEM 20°C (days) 75th percentile 20°C (days) p value against control n No.of Exp
N2 Ex[rol-6] 19.57 ± 0.2 21 - 129/130 2
N2 Ex008 [SKN-1 S393A:: GFP] 2.5ng/µl 23.62 ± 0.3 26 <.0001a 78/78 2
N2 Ex021 [SKN-1 S393A:: GFP] 2.5ng/µl 21.80 ± 0.2 23 <.0001a 117/122 2
N2 Ex022 [SKN-1 S393A:: GFP] 2.5ng/µl 21.10 ± 0.3 23 <.0001a 62/62 2
N2 Ex023 [SKN-1 S393A:: GFP] 2.5ng/µl 20.89 ± 0.3 23 <.0001a 96/96 2
N2 Ex024 [SKN-1 S393A:: GFP] 2.5ng/µl 20.66 ± 0.4 23 <.0001a 63/63 2
N2 Ex[rol-6] 19.52 ± 0.2 21 - 236/243 3
skn-1(zu67) Ex[rol-6] 15.51 ± 0.3 18 <0.0001a 96/96 2
N2 Ex001[SKN -1B/C:: GFP] 2.5ng/µl 22.14 ± 0.3 25 <.0001b,c 146/148 2
skn-1(zu67) Ex001[SKN -1B/C:: GFP] 2.5ng/µl 23.53 ± 0.6 26 <.0001b,c 48/51 2
skn-1(zu67) Ex008[SKN -1B/C S393A:: GFP] 2.5ng/µl 25.74 ± 0.5 29 <.0001b,c 67/70 2
skn-1(zu67) Ex020[SKN -1B/C S393A:: GFP] 10ng/µl 27.16 ± 0.6 29 <.0001b,c 30/40 1
N2 Ex[rol-6]; control(RNAi) 20.60 ± 0.5 23 - 50/50 1
N2 Ex[rol-6]; skn-1(RNAi) 19.91 ± 0.4 21 0.0699f 60/60 1
N2 Ex[rol-6]; skn-1a/c(RNAi) 20.37 ± 0.4 23 0.7880f 59/60 1
skn-1(zu67) Ex001[SKN -1B/C:: GFP] 2.5ng/µl ;control(RNAi) 24.20 ± 0.4 27 <.0001f 62/64 1
skn-1(zu67) Ex001[SKN -1B/C:: GFP] 2.5ng/µl;skn-1(RNAi) 21.58 ± 0.4 23 <.0001d 84/84 1
skn-1(zu67) Ex001[SKN -1B/C:: GFP] 2.5ng/µl ;skn-1a/c(RNAi) 21.06 ± 0.5 25 0.0005d 69/69 1
N2 Ex[rol-6] 20.60±0.5 23 - 50/50 1
daf-16(mgDf47) 15.87±0.3 17 <.0001f 58/61 1
daf-16(mgDf47) Ex008[SKN-1 S393A:: GFP] 2.5ng/µl 18.88±0.3 21 <.0001e 48/48 1
N2 Ex[rol-6]; control(RNAi) 20.02 ± 0.2 22 - 256/259 4
N2 Ex[rol-6]; daf-16(RNAi) 17.77 ± 0.1 19 <.0001f 266/266 4
skn-1(zu67) Ex[rol-6]; control(RNAi) 17.38 ± 0.3 20 <.0001f 145/146 3
skn-1(zu67) Ex[rol-6]; daf-16(RNAi) 16.59 ± 0.3 19 0.0240g 142/145 3
skn-1(zu67)Ex001[SKN-1b/c:: GFP] 2.5ng/µl; control(RNAi) 22.95 ± 0.5 26 <.0001g 62/62 2
skn-1(zu67) Ex001[SKN-1b/c:: GFP] 2.5ng/µl; daf-16(RNAi) 16.53 ± 0.3 18 0.1655h 59/59 2
skn-1(zu67) Ex008[SKN-1 S393A:: GFP] 2.5ng/µl; control(RNAi) 24.28 ± 0.6 28 <.0001g 64/76 3
skn-1(zu67) Ex008[SKN-1 S393A:: GFP] 2.5ng/µl; daf-16(RNAi) 21.12 ± 0.4 23 <.0001h,i 76/87 3
skn-1(zu67) Ex020[SKN-1 S393A:: GFP] 10ng/µl; control(RNAi) 25.10 ± 0.6 28 <.0001g 121/132 3
skn-1(zu67) Ex020[SKN-1 S393A:: GFP] 10ng/µl; daf-16(RNAi) 21.39 ± 0.4 24 <.0001h,i 124/140 3

Corresponds to the data in Figure 5A–E. p values were calculated as follows:

a

N2 Ex[rol-6],

b

skn-1(zu67)[rol-6],

c

significantly enhanced compared to N2 [rol-6] (p=<0.0001 in each case),

d

skn-1(zu67) Ex001 control(RNAi),

e

daf-16(mgDf47) control(RNAi),

f

N2 Ex[rol-6] control(RNAi),

g

skn-1(zu67)[rol-6] control(RNAi),

h

skn-1(zu67)[rol-6] daf-16(RNAi),

i

Compared to N2 Ex[rol-6] control(RNAi) (p=0.0021 for skn-1(zu67) Ex008 and p=0.0001 for skn-1(zu67) Ex020). Individual experiments and additional analysis of daf-16 Ex008[SKN-1S393A::GFP] are in Table S6. Tabulated as in Table 1.