Table 3.
Differential P. multocida protein expression in response to a quarter-MIC AMX, CTC, and ENR in different GO categories.
| Accession | GO Slims | ∑Xcorr | |||
|---|---|---|---|---|---|
| Pm70 | AMX | CTC | ENR | ||
| Glycolysis/Gluconeogenesis | |||||
| 15603407 | Phosphoenolpyruvate carboxykinase, PckA | 264.2 | ns | 120 | 112.9 |
| 15603725 | Phosphoglycerate kinase, Pgk | 218.1 | ns | 170.2 | 161 |
| 15603726 | Fructose-bisphosphate aldolase, FbaA | 504.9 | 566.6 | 450.1 | 436.8 |
| 15602789 | Glyceraldehyde-3-phosphate dehydrogenase, GapA | 253.6 | ns | 125.5 | 70.1 |
| 15603371 | Phosphoglyceromutase, GpmA | 98.3 | 124.9 | 130.3 | ns |
| 15602518 | Pyruvate kinase, PykA | 28.6 | 51.4 | 54.9 | ns |
| 15602688 | Fumarate hydratase, FumC | 42.2 | 80.5 | 19.1 | ns |
| 15602146 | SucD | bdt | 7.8 | ns | ns |
|
| |||||
| Response to stress | |||||
| 15602972 | Chaperonin GroEL | 148.6 | 246.8 | ns | 177.1 |
| 15602971 | Co-chaperonin GroES | 23 | 42.7 | 56.3 | 56.6 |
| 15602601 | DnaK | 97.6 | 132.7 | ns | 72.6 |
| 15602303 | FtsH | 21.9 | 7.9 | 4.7 | 3.9 |
|
| |||||
| Peptidoglycon biosynthesis and cell envelope permeability | |||||
| 15603596 | D-fructose-6-phosphate amidotransferase, GlmS | bdt | 31.3 | 12.4 | 6.8 |
| 15603671 | GlmU | 8.5 | 20.2 | ns | ns |
| 15602548 | NagC | 14.4 | ns | 5.3 | 5 |
| 15602651 | Hypothetical protein PM0786, OmpA | 262.8 | ns | 172.5 | 76 |
| 15602419 | Lpp | 7.2 | ns | 18.7 | ns |
|
| |||||
| Purine metabolism | |||||
| 15602149 | Adenylate kinase, Adk | 2.8 | 22.3 | 42.2 | 11.3 |
| 15602803 | Adenylosuccinate synthetase, PurA | 2.4 | ns | 11.2 | 19.2 |
| 15602109 | Ribose-phosphate pyrophosphokinase, PrsA | bdt | 9.2 | 8.4 | ns |
|
| |||||
| RNA metabolism | |||||
| 15603255 | DNA-directed RNA polymerase alpha subunit, RpoA | 32.8 | 76.1 | 51.9 | ns |
| 15603602 | DNA-directed RNA polymerase beta subunit, RpoB | 17 | 34.5 | ns | ns |
| 15603601 | DNA-directed RNA polymerase beta~ subunit, RpoC | 56.2 | 85.2 | ns | ns |
| 15602786 | DNA-directed RNA polymerase omega subunit, RpoZ | bdt | 11 | ns | 9.6 |
| 15602119 | Lrp | 8.7 | ns | 25.1 | ns |
| 15603209 | DeoR | bdt | 10.6 | ns | ns |
| 15603785 | Transcription termination factor Rho | 42.4 | 15.9 | 20.5 | 21.3 |
|
| |||||
| Xenobiotic metabolism | |||||
| 15601866 | SodA | 153.5 | ns | 66 | 85.4 |
| 15601897 | HktE/KatE | 26.1 | ns | 3 | 6.1 |
|
| |||||
| Lipopolysaccharide biosynthesis | |||||
| 15603862 | Lipid-A-disaccharide synthase, LpxB | bdt | 12.1 | ns | ns |
| 15602423 | 2-dehydro-3-deoxyphosphooctonate aldolase, KdsA | 9.2 | 23.2 | ns | ns |
| 15602749 | RfaE | bdt | 12.9 | ns | ns |
| 15603154 | GalU | 3.7 | 18.7 | ns | ns |
|
| |||||
| Cell division | |||||
| 15603384 | FtsY | bdt | 8.2 | ns | ns |
| 15602012 | Cell division protein FtsZ | 11.5 | 22.1 | ns | ns |
|
| |||||
| Protein translation | |||||
| 15603222 | Elongation factor Tu | 875.7 | ns | 936 | ns |
| 15602977 | DeaD | 4.7 | ns | 13.3 | ns |
| 15602168 | Methionyl-tRNA synthetase | 2.4 | ns | 11.5 | ns |
|
| |||||
| DNA repair | |||||
| 15603682 | Recombinase A, RecA | bdt | ns | ns | 19.5 |
AMX: amoxicillin; CTC: chlortetracycline; ENR: enrofloxacin.
Pm70: P. multocida cultured without sub-MICs.
bdt: below detectable threshold.
ns: differential expression not significant compared to Pm70.