Figure 4.
CD24+CD29+ cells maintain their ability to differentiate. CD24+CD29+ cells were sorted out from the W0069 cell line and they were cultured as monolayers. After 3 passages, flow cytometry analysis was performed using anti CD24-PE and anti CD29-FITC (A) or anti CD24-PE and anti 49f-FITC (B). Same strategy was followed in the case of CD24-CD29- sorted out cells (C, D). Representative data after flow cytometry are shown. (E) morphology of double positive or double negative cell in culture under microscope.