Table 1. Criteria for the prediction of a dual-targeted mitochondrial proteome.

Protein localization	Database type	Methodology	Reference
1st location: Mitochondria Reference set	MitoP2	Single gene study	http://www.mitop.de
1st location: Mitochondria MitoP2	MitoP2 SVM (support vector machine)	MitoP2 SVM >0.5	http://www.mitop.de
1st location: Mitochondria Simple evaluation	Mitochondrial proteomics	Mitochondria purification +2D PAGE / Mass Spectrometry	Sickmann et al 2003
	Subcellular localization screens	Chromosomally GFP-tagged Proteins	Huh et al 2003
		Transposon-Insertion Phenotypes, Localization and Expression (TRIPLE)	Kumar et al 2002
	Null mutant phenotype	Pet mutants	Dimmer KS et al 2002
	In-Silico predictions	MTS prediction – MitoProtII >0.7	http://ihg.gsf.de/ihg/mitoprot.html
		Homology to the prokaryotic ortholog starts after 10–80 amino acids	blast
2nd location	Subcellular localization screens	Chromosomally GFP-tagged Proteins	Huh et al 2003
		Transposon-Insertion Phenotypes, Localization and Expression (TRIPLE)	Kumar et al 2002
	In-Silico predictions	Peroxisome targeting sequence prediction – PTS1 predictor	http://mendel.imp.univie.ac.at/mendeljsp/sat/pts1/PTS1predictor.jsp
		ER signal peptide prediction - TargetP	http://www.cbs.dtu.dk/services/