Table 3. Comparison of MTS parameters of dual localized versus exclusive mitochondrial proteins in the mitochondrial reference set.

Parameter	Protein location	N	Median	Mean	SD	p-value* (Mann-Whitney)	p-value* (χ2 score, df)
μHδ	Mitochondrial proteins	535	7.064	6.856	2.578	-	-
	Predicted exclusive mitochondrial proteins	409	7.387	7.099	2.46	<0.001	<0.0026 (25.3, 9)
	Predicted dual localized mitochondrial proteins	126	5.905	6.064	2.79
Hmax	Mitochondrial proteins	535	4.61	4.431	1.72	-	-
	Predicted exclusive mitochondrial proteins	409	4.71	4.521	1.62	0.06	0.11 (10.2, 6)
	Predicted dual localized mitochondrial proteins	126	4.385	4.136	1.98
Number of positively charged residues in N-terminus	Mitochondrial proteins	535	6.00	5.94	4.189	-	-
	Predicted exclusive mitochondrial proteins	409	6.00	6.22	4.16	0.005	0.01 (27.5, 13)
	Predicted dual localized mitochondrial proteins	126	5.00	5.02	4.15

The hydrophobic moment (μHδ), maxmimal hydrophobicity (Hmax), and the number of positive charged residues in the N-terminus are parameters used to evaluate the strength of mitochondrial targeting sequences (MTS, see text). Statistical analysis of differences between parameters of dual and exclusive mitochondrial proteins was carried out as in Table 2.