Table 4. Comparison of the total net charge of dual localized versus exclusive mitochondrial proteins of the reference set.

Protein location	Total Net Charge
	N	Median	Mean	SD	p-value* (Mann-Whitney)	p-value* (χ2 score, df)
Mitochondrial proteins	535	6.00	6.80	11.82	-	-
Predicted exclusive mitochondrial proteins	409	7.00	7.29	10.51	0.0024	0.032 (10.6, 4)
Predicted dual localized mitochondrial proteins	126	3.00	5.21	15.26

Dual targeted mitochondrial proteins have a lower total protein net charge than exclusive mitochondrial proteins. Statistical analysis of differences between parameters of dual and exclusive mitochondrial proteins was carried out as in Table 2.^* Differences are considered significant if p-value <0.05.