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. 1998 Oct 27;95(22):12973–12976. doi: 10.1073/pnas.95.22.12973

Figure 4.

Figure 4

N-terminal part of GEF115 contains a RGS motif. (A) NIH 3T3 cells were cotransfected with 0.15 μg of SRE.L-luciferase reporter plasmid, 0.15 μg of GFP expression construct, and 0.2 μg of LacZ, Axin or RGS12 in the presence or absence of 0.05 μg of wild-type Gα12 or Gα13 subunits. One day later cells were lysed, and GFP levels and luciferase activity were determined as in Fig. 1. (B) NIH 3T3 cells were cotransfected with 0.15 μg of CRE-luciferase reporter plasmid, 0.15 μg of GFP expression construct, and 0.2 μg of LacZ, GEF115ΔDH or RGS expression plasmids. Isoprenaline (10 μM) was added the next day for 6 hr. Then GFP levels and luciferase activity were determined as in Fig. 1. (C) The amino acid sequences of GEF115 and RGS12 were compared by using a computer program macaw. Homologous regions were shown. Identical amino acids between RGS12 and GEF115 are shown in boxes.