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Fluorescent immunohistochemical double labelling for β-amyloid and IL-1β. Representative muscle biopsy specimen of a patient with sIBM stained with anti-β-amyloid (Alexa-488, green; left) and anti-IL-1β (Alexa-594, red; middle). A degenerating muscle fibre in the centre is double positive (arrows; yellow in merged image, right). Photos taken by a CCD camera using a conventional fluorescent microscope with a 40× objective. Scale bar represents 40 µm.
